اندازه‌گیری فعالیت آدنیلیل سیکلاز غشاء سلولی در حضور پروتئین کموتاکسیک ماکروفاژ

 Adenylyl cyclase is a membrane-bound enzyme that catalyzes the conversion of ATP to cAMP. The inhibition of adenylyl cyclase was carried out by measuring the ability of the macrophage chemotactic protein-1 to inhibit the forskolin-induced enzyme activity. Measurement of adenylyl cyclase activity was performed according to the procedure described by Wiegn.  Adenylyl cyclase activity in the present macrophage chemotactic protein-1 was decreased compared to that in controls [2.11+/- 0.15(mean +/-SD.)vs. 6.83+/-0.45, activity ( µ mol cAMP/mg protein/min)].  Macrophage chemotactic protein significant reduced adenylyl cyclase activity by 79.9%.